{"id":2256,"date":"2019-08-05T08:47:19","date_gmt":"2019-08-05T06:47:19","guid":{"rendered":"https:\/\/www.e-spectations.com\/?p=2256"},"modified":"2021-11-15T15:07:49","modified_gmt":"2021-11-15T13:07:49","slug":"antibody-production","status":"publish","type":"post","link":"https:\/\/e-spectations.com\/portfolio\/antibody-production\/","title":{"rendered":"Antibody production"},"content":{"rendered":"\n
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Antibody production<\/h3>

An overview of antibody production<\/p><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section>\n\n\n\n

Table of Contents<\/div><\/div><\/div>
  1. Antibody Production – background<\/a>
    1. Planning Antibody Production:<\/a><\/li>
    2. Antibody Purification<\/a><\/li>
    3. How to characterize antibodies:<\/a><\/li>
    4. Antibody Fragmentation<\/a><\/li>
    5. Labelling and Immobilization:<\/a>
      1. Conclusion:<\/a>
        1. References:<\/a><\/li><\/ol><\/li><\/ol><\/li><\/ol><\/li><\/ol><\/div><\/div><\/div><\/section>\n\n\n\n

          Antibody Production – background<\/h2><\/div><\/div>\n\n\n\n

          In the 1970s-1980s, scientists developed procedures to generate, purify and modify antibodies for use as antigen-specific probes.  

          In 1988 Harlow and Lane publish a manual (          Antibodies: A Laboratory manua<\/a>l) standardizing protocol.  These protocols are still in use today. Not much has changed as far as methods are concerned.

          Animal immune systems harness the ability to produce antibodies – these antibodies have the ability to bind to specific antigens, therefore creating an opportunity for research and development scientists to manufacture probes for specific molecule detection.

          Antibodies’ protein structure is relatively uniform.  This uniformity enables antibody purification, labelling, and detection in a predictable and reproducible method.

          In this article, we take a look at the processes required during antibody production.<\/p><\/div><\/div>\n\n\n\n

          \"Antibody